ABSTRACT
Objective: To study the chemical constituents of the roots of Swertia kingii. Methods: The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 chromatographies. Their structures were identified on the basis of physicochemical properties and spectrometric data. Results: Ten compounds obtained from this plant were identified as demethylbellidifolin (1), β-sitosterol (2), 1,3,7-trihydro-xanthone (3), swertianolin (4), 1,3,7-trihydrox-8-methoxy-xanthone (5), 1,5,8- trihydroxy-3-methoxy-xanthone (6), 1,3,7,8-tetrahydroxyxanthone-1-O-β-D-glucopyranoside (7), 1,3,7,8-tetrahydroxy-xanthone (8), 1-hydroxy-3,7,8-trimethoxy-xanthone (9), and 1,2,6,8-tetrahydroxy-xanthone (10). Conclusion: Except for compound 2, the other compounds are obtained from this plant for the first time.
ABSTRACT
Objective To establish an HPLC method for simultaneously determining eight components, such as sweroside, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone in the root of Swertia kingii. Methods Chromatographic analysis was achieved on an Agilent Zorbax ODS column (250 mm × 4.6 mm, 5 μm) by gradient elution of acetonitrile-0.5% phosphoric acid in water at 30 ℃. The flow rate was 1.0 mL/min and the detection wavelength was 254 nm. Results The calibration curves of all the eight constituents showed good linearity in a relatively wide concentration range. The linear ranges of swertiamarin, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone were 3.84-96.00 μg/mL (r = 0.999 6), 3.36-84.00 μg/mL (r = 0.999 2), 5.92-148.00 μg/mL (r = 0.999 8), 4.81-118.00 μg/mL (r = 0.999 2), 4.32-108.00 μg/mL (r = 0.999 3), 4.16-104.00 μg/mL (r = 0.999 2), 5.12-128.00 μg/mL (r = 0.999 4), 4.80-120.00 μg/mL (r = 0.999 6), respectively. The average recoveries were 99.59% (RSD 0.99%), 98.95% (RSD 3.37%), 98.61% (RSD 1.87%), 99.63% (RSD 1.93%), 99.31% (RSD 1.21%), 99.50% (RSD 1.62%), 99.80% (RSD 0.54%), and 98.50% (RSD 1.87%). Conclusion This method is simple, efficient, accurate, and reproducible, and can be used for the determination of eight constituents in root of Swertia kingii. This will promote the comprehensive usage of this plant.